2019-nCoV Te Whakamaatau Whakamatau Antibody （QDIC）
Taipitopito Hua:
Ko te Innovita® 2019-nCoV IgM/IgG Whakamātautau he tikanga mo te kimi inenga o te whakakore i te antibody ki te coronavirus hou (2019-nCoV) i roto i nga tauira serum tangata, plasma, toto katoa ranei (toto maihao, toto toto katoa ranei).
Kei roto i te 2019-nCoV e wha nga pūmua hanganga matua: pūmua S, pūmua E, pūmua M me pūmua N.Ka taea e te rohe RBD o te pūmua S te here ki te kaikawe mata o te tangata ACE2.Kua whakaatuhia e nga rangahau ko nga tauira o nga tangata kua ora mai i te mate coronavirus hou he pai mo te whakakore i te antibody.Ka taea te whakamahi i te kitenga o te aukati antibody ki te aromatawai i te matapaetanga o te mate huaketo me te arotakenga paanga i muri i te werohanga.
Kaupapa:
Ko te kete ko te quantum dot immunofluorescence chromatography assay ki te kite i te 2019-nCoV RBD motuhake IgG e whakakore ana i nga paturopi i roto i te serum tangata, te plasma, te toto katoa ranei (toto maihao me te toto venous katoa) tauira.I muri i te hoatutanga o te tauira ki te pai o te tauira, mena he teitei ake te kukū o te aukati iraro paturopi i te iti rawa o te rohenga kitea, ka tauhohe nga RBD motuhake IgG antigen ki tetahi waahanga, RBD katoa ranei kua tapaina ki nga microspheres quantum dot hei hanga i te pūhui aukati.Na ka heke te pūhui ārai mate i te taha o te kiriuhi nitrocellulose.Ka tae ki te rohe whakamatautau (raina T), ka tauhohe te pūhui ki te kiore anti-tangata IgG (meiini γ) kua pania ki runga i te kiriuhi nitrocellulose ka hanga i te raina rewharewha.Panuitia te uara tohu rewharewha me te kaitirotiro immunoassay fluorescence.Ko te uara tohu he rite ki te ihirangi o te whakakore i nga paturopi i roto i te tauira.
Ahakoa kei roto i te tauira he RBD nga patu paturopi aukati motuhake, kaore ranei, me puta tonu te raina mana ki te matapihi hua mena ka tika te mahi whakamatautau ka mahi te reagent i runga i te whakaaro.Ina heke te heihei IgY antibody kua tapaina ki te quantum dot microspheres ki te raina whakahaere (raina C), ka mauhia e te koati anti-chicken antibody IgY kua pania ki runga i te raina C, a ka hanga he raina rewharewha.Ko te raina mana (C line) ka whakamahia hei mana whakahaere.
Tito:
| Te tito | Te nui | Whakatakotoranga |
| IFU | 1 | / |
| Ripene whakamatautau | 20 | Ia pukoro takai hiri kei roto kotahi te taputapu whakamatautau me tetahi whakamaroke |
| Tauira waimeha | 3mL*1 ipu | 20mM PBS, Sodium Casein, ProClin 300 |
| Micropipette | 20 | Micropipette me te raina tohu 20μL |
| Lancet | 20 | / |
| Papa waipiro | 20 | / |
Te Tikanga Whakamatau:
● Kohinga Toto Matimati
● Pānuihia te hua me te kaitātari fluorescence
