Hua

Taipitopito Hua:

Ko te Innovita® 2019-nCoV IgM/IgG Whakamātautau he tikanga mo te rapunga inenga-a-ira mo te whakakore i nga paturopi ki te coronavirus hou (2019-nCoV) i roto i te serum tangata, te plasma, nga tauira toto katoa ranei.
Kei roto i te 2019-nCoV e wha nga pūmua hanganga matua: pūmua S, pūmua E, pūmua M me pūmua N.Ka taea e te rohe RBD o te pūmua S te here ki te kaikawe mata o te tangata ACE2.Ko te whakakore i te antibody e pa ana ki te kaha ki te here ki te tukumate, katahi ka aukati i te tukumate ki te uru ki te tinana kia mate ai.Ka taea te whakamahi i te kitenga o te aukati antibody ki te aromatawai i te matapaetanga o te mate huaketo.

Kaupapa:

Ko te kete he whakamatautau whakataetae immunochromatography koura colloid ki te kite i te whakakore i nga paturopi ki te 2019-nCoV i roto i te serum tangata, plasma, tauira toto katoa ranei.Whai muri i te hoatutanga o te tauira ki te pai o te tauira, mena kei te noho nga paturopi whakaheke i roto i te tauira, ka tauhohe nga paturopi whakaheke ki te koura colloidal kua tapaina te RBD antigen hei hanga i te matatini aukati, a ka kati te waahi whakaheke o te RBD antigen kua tapaina.Kātahi ka heke te matatini ārai mate me te antigen RBD kua tapaina me te kore e herea ki te whakakore i te antibody ki te taha o te membrane nitrocellulose.Ka tae ki te rohe whakamatautau (raina T), ka tauhohe te antigen RBD kua tapaina me te kore e herea ki te whakakore i nga paturopi me te antigen ACE2 kua pania ki te kiriuhi nitrocellulose ka hanga he raina papura-whero.Ki te teitei ake te kukū o te whakakore i nga antibodies i te iti rawa o te kitenga, he mama ake te raina papura-whero i te raina whakahaere (raina C) karekau ranei he raina papura-whero i hanga, he pai te hua.Ina he iti ake te kukū o te aukati i te parekuihi i te iti rawa o te kitenga, karekau ranei he paturopi whakaheke i roto i te tauira, he pouri ake te raina papura-whero i te raina whakahaere, he kino te hua.
Ahakoa kei roto i te tauira nga paturopi 2019-nCoV, i te wa ka heke te karaehe IgY heihei tohu koura ki te raina whakahaere (raina C), ka mauhia e te koati anti-moa IgY antibody kua pania ki runga i te raina whakahaere (C). raina), ka hangaia he raina papura-whero.Ko te raina mana (C line) ka whakamahia hei mana whakahaere.Me puta tonu nga raina mana ki nga matapihi hua mena ka mahia tika te tikanga whakamatautau ka mahi nga reagents i runga i te whakaaro.

Tito:

Te Tikanga Whakamatau:

1. Wewetehia te putea konumohe konumohe ka tango i te rīpene whakamātautau.
2. Whakamahia te 40μL o te tauira serum/plasma ranei 60μL tauira toto katoa ki te pai tauira.
3. Hoatuhia te 40μL (2 pata) tauira waimeha ki te puna tauira.
4. Whakanohoia ki te pāmahana rūma (15 ℃ ~ 30 ℃) mo te 15-20 meneti, ka panuihia te hua.

Hua Whakamaori:

1. He pai: Ina he mama ake te tae o te raina T i tera o te raina C, ki te kore ranei he raina T, he tohu pai mo te whakakore i nga paturopi.
2. He kino: Ina he pouri ake te tae o te raina T i te rite ranei ki tera o te raina C, he tohu toraro mo te whakakore i nga antibodies.
3. Muhu: Ki te kore e puta te raina C, ahakoa ka kitea te raina T, kaore ranei, ka muhu te whakamatautau.Whakatauhia te whakamatautau me tetahi whakamatautau hou.